One of the most challenging studies this group has ever completed. What started out as a simple idea required years of improvement in instrumentation, completely new approaches to data analysis and the development of a new model to understand how actin polymerises. We think a first in terms of demonstrating what can be done with MP in the context of highly heterogeneous mixtures.

We review recent advances in our ability to characterise biomolecular structure, interactions and associated dynamics by mass photometry (MP), the label-free detection and mass measurement of individual biomolecules in solution. Molecular counting and identification provides direct access to relative abundance, and thereby affinities, while associated dynamics yield on- and off-rates. The molecular resolution afforded by MP enables these measurements as a function of stoichiometry and assembly at equilibrium, as opposed to the majority of existing solution-based methods. Together with future improvements in terms of assays and technological performance, MP is likely to provide mechanistic details of complex biomolecular processes.

A collaboration that started on the blackboard after a talk in Bielefeld, involved a number of visits by Michael to Oxford and resulted in a great example how mass photometry can help with understanding polydispersity of proteins, and how that polydispersity relates to function.

It's not all about interferometric scattering. Darkfield, especially with super-optimised micromirrors is really, really awesome. If you are tracking metal nanoparticles, we think this is the way to go. And maybe even for molecules...

Wonderful work by the He group on designing protein nanoparticles to which we could contribute with mass photometry-based characterisation of protein oligomerisation.

Mass photometry unchained from microscope cover glass. Detection, imaging, tracking and mass measurement of membrane-associated complexes. In our opinion, the beginning of a new era for MP.

Jack and Lee really, really went all the way in terms of trying to provide an overview of scattering-based light microscopy on the nanoscale. Apologies to those we missed, but hopefully a good starting point for people getting going in this exciting field.

Yet another collaboration that started with "let's just have a look" where we could contribute to understanding the role of protein oligomerisation in R2TP with mass photometry.

An overview of light-based, label-free methods for studying how biomolecules interact. Our contribution is of course MP, but lots of amazing methods out there!

Started with a 5 minute chat after listening to Tim's amazing talk at the Klosters Winterseminar, and turned into an amazing story. Huge amount of work by the Clausen lab, with us contributing olgiomerisation dynamics - and surprises - at low concentration.

Just one of the papers the emerged from a sabbatical Sanli Faez from Uetrecht spent in Oxford - this one using single DNA molecules with gold particles attached to sense force on the nanoscale.

Another collaboration with IMP Vienna on proteasomal degradation mechanisms where we used mass photometry to help determine the stoichiometry and oligomerisation of LUBAC.